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Avicenna Journal of Medical Biochemistry، جلد ۴، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
Neural Differentiation of Human Umbilical Cord Blood-derived Mesenchymal Stem Cells |
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| چکیده فارسی مقاله |
Conclusions Taken together, the combination of chemical and growth factors in the two-step induction protocol may improve the efficiency of MSC differentiation into neural progenitor cells, and may be a new method for the easy and fast application of MSCs in regenerative medicine in the future. Results The results of real-time PCR showed that the expression of the GFAP, MBP, and MAP-2 genes after two-step induction was significantly increased compared to the common induction protocol. In addition, our study showed that RA should play the main role in the neural differentiation and fate of MSCs compared to other neural inducers. Materials and Methods MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator equilibration at 5% CO2 and 37°C. For the neural differentiation of MSCs, the DMEM was removed and replaced with pre-induction media (retinoic acid [RA], basic fibroblast growth factor [bFGF], and epidermal growth factor [EGF]) and basal medium for two days. They were then cultured in nerve growth factor (NGF), 3-isobutyl-1-methylxanthine (IBMX), ascorbic acid (AA), and basal medium for six days. We also monitored the expression of markers for neural differentiation with real-time PCR. Background Cell therapy is a potential therapeutic approach for neurodegenerative disorders. Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) are an appropriate source of stem cells for use in various cell-based therapies. Objectives In this study, we examined a real-time PCR approach for neural differentiation of hUCB-MSCs in vitro. |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Neural Differentiation of Human Umbilical Cord Blood-derived Mesenchymal Stem Cells |
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| چکیده انگلیسی مقاله |
Conclusions Taken together, the combination of chemical and growth factors in the two-step induction protocol may improve the efficiency of MSC differentiation into neural progenitor cells, and may be a new method for the easy and fast application of MSCs in regenerative medicine in the future. Results The results of real-time PCR showed that the expression of the GFAP, MBP, and MAP-2 genes after two-step induction was significantly increased compared to the common induction protocol. In addition, our study showed that RA should play the main role in the neural differentiation and fate of MSCs compared to other neural inducers. Materials and Methods MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator equilibration at 5% CO2 and 37°C. For the neural differentiation of MSCs, the DMEM was removed and replaced with pre-induction media (retinoic acid [RA], basic fibroblast growth factor [bFGF], and epidermal growth factor [EGF]) and basal medium for two days. They were then cultured in nerve growth factor (NGF), 3-isobutyl-1-methylxanthine (IBMX), ascorbic acid (AA), and basal medium for six days. We also monitored the expression of markers for neural differentiation with real-time PCR. Background Cell therapy is a potential therapeutic approach for neurodegenerative disorders. Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) are an appropriate source of stem cells for use in various cell-based therapies. Objectives In this study, we examined a real-time PCR approach for neural differentiation of hUCB-MSCs in vitro. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
حسن رفیعی مهر | hassan rafieemehr
department of medical laboratory sciences, school of para-medicine, hamadan university of medical sciences, hamadan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
مریم خیراندیش | maryam kheirandish
department of immunology, blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran; department of immunology, blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran. tel 98-218860150130, fax 98-2188601555
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
مسعود سلیمانی | masoud soleimani
department of hematology, faculty of medicine, tarbiat modares university, tehran, ir iran; department of stem cell biology, stem cell technology research center, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=29066 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
10.17795/ajmb-29066 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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