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Avicenna Journal of Medical Biochemistry، جلد ۴، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
Cytotoxic Effects of Some Common Organic Solvents on MCF-۷, RAW-۲۶۴.۷ and Human Umbilical Vein Endothelial Cells |
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| چکیده فارسی مقاله |
Background Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium. |
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| کلیدواژه‌های فارسی مقاله |
Cytotoxicity،MCF-7،RAW-264.7،HUVEC،Organic Solvents |
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| عنوان انگلیسی |
Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells |
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| چکیده انگلیسی مقاله |
Background Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium. |
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| کلیدواژه‌های انگلیسی مقاله |
Cytotoxicity,MCF-7,RAW-264.7,HUVEC,Organic Solvents |
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| نویسندگان مقاله |
لیلا جمالزاده | leila jamalzadeh
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
حسین غفوری | hosein ghafoori
department of biology, university of guilan, rasht, ir iran; department of biology, university of guilan, rasht, ir iran. tel fax 98-1333333647
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
ریحانه سریری | reyhaneh sariri
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
هانیه rabuti | hanieh rabuti
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
ژیلا نصیرزاده | jila nasirzade
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
هاجر حسنی | hajar hasani
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
محمودرضا آقا معالی | mahmoud reza aghamaali
department of biology, university of guilan, rasht, ir iran
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=33453 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
10.17795/ajmb-33453 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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