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Avicenna Journal of Medical Biochemistry، جلد ۲، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
High Molecular Weight Alkaline Phosphatase Changes Following Animal Copper Treatment |
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| چکیده فارسی مقاله |
Conclusions The elevation of HMW-ALP activity in Cu treated animal suggests the occurrence of biliary disease. This may be used as a biomarker for the diagnosis of copper toxicity. Results Obtained data showed that with increasing administration of copper, the ALP activity was elevated significantly. In comparison with the control group the elevations were between 20%-56% using gel filtration chromatography. It was found that the elevation of serum ALP was mostly due to HMW-ALP. Objectives This study aimed to investigate the relationship between copper treatment and changes in the serum concentration of high molecular weight alkaline phosphatase (HMW-ALP). Materials and Methods Male Wistar rats were injected intraperitoneally (IP) with copper (Cu) as copper chloride (CuCl2. 4H2O) 4, 2 and 1 mg/kg for 10, 30 and 60 days respectively. Animals were killed at indicated time and blood samples were collected, and sera was separated and used for alkaline phosphatase activity determinations and also for isoenzymes gel filtration chromatography and Sephacryl S-300 was used. Background Although trace amounts of copper (Cu) are necessary to maintain proper body functions, the excess amount can contribute to the development of hepatic dysfunction. |
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| کلیدواژه‌های فارسی مقاله |
Alkaline Phosphatase،Copper،Liver |
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| عنوان انگلیسی |
High Molecular Weight Alkaline Phosphatase Changes Following Animal Copper Treatment |
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| چکیده انگلیسی مقاله |
Conclusions The elevation of HMW-ALP activity in Cu treated animal suggests the occurrence of biliary disease. This may be used as a biomarker for the diagnosis of copper toxicity. Results Obtained data showed that with increasing administration of copper, the ALP activity was elevated significantly. In comparison with the control group the elevations were between 20%-56% using gel filtration chromatography. It was found that the elevation of serum ALP was mostly due to HMW-ALP. Objectives This study aimed to investigate the relationship between copper treatment and changes in the serum concentration of high molecular weight alkaline phosphatase (HMW-ALP). Materials and Methods Male Wistar rats were injected intraperitoneally (IP) with copper (Cu) as copper chloride (CuCl2. 4H2O) 4, 2 and 1 mg/kg for 10, 30 and 60 days respectively. Animals were killed at indicated time and blood samples were collected, and sera was separated and used for alkaline phosphatase activity determinations and also for isoenzymes gel filtration chromatography and Sephacryl S-300 was used. Background Although trace amounts of copper (Cu) are necessary to maintain proper body functions, the excess amount can contribute to the development of hepatic dysfunction. |
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| کلیدواژه‌های انگلیسی مقاله |
Alkaline Phosphatase,Copper,Liver |
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| نویسندگان مقاله |
جمشید کریمی | jamshid karimi
department of biochemistry and nutrition, hamadan university of medical sciences, hamadan, ir iran; department of biochemistry and nutrition, hamadan university of medical sciences, hamadan, ir iran. , 98-8138380462
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
علی اصغر مشتاقی | ali asghar moshtaghie
department of biochemistry, isfahan university of medical sciences, isfahan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
بهرام حقیقی | bahram haghighi
department of biochemistry, isfahan university of medical sciences, isfahan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=18255 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
10.17795/ajmb-18255 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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