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Avicenna Journal of Clinical Microbiology and Infection، جلد ۳، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
Correlation Between ISAba۱ Upstream ampC Gene and Resistance to Cefotaxime in Acinetobacter baumannii: A Serious Threat to Nosocomial Infections |
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| چکیده فارسی مقاله |
Conclusions ISAba1 has become an important factor in A. baumannii’s resistance to cefotaxime. Results ISAba1 located upstream of blaampC was found in 24 (48%) of the A. baumannii isolates. In all of the studied isolates that had ISAmpC, the MIC for cefotaxime was 64 - 256 μg/mL. Based on the REP-PCR patterns among the resistant isolates, the highest number of ISAmpC positive isolates belonged to type B (n = 19) and type C (n = 12). Objectives We examined the prevalence ISAmpC and its correlation with cefotaxime resistance. Materials and Methods Standard biochemical tests were used to identify isolates. Genomic species of the genus Acinetobacter were confirmed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). The susceptibility of 50 A. baumannii isolates to a variety of antimicrobial agents was determined using the disk diffusion method and E-test strips. PCR was used to investigate the connection of insertion sequences and the ampC gene. Clonal relatedness was determined by Repetitive Extragenic Palindromic PCR. Background Infections due to Acinetobacter baumannii have become a significant challenge in modern healthcare systems. The global upsurge of multidrug resistance in A. baumannii has created widespread problems in the treatment of patients. |
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| کلیدواژه‌های فارسی مقاله |
ISAba1،ampC،Nosocomial Infection،Acinetobacter baumannii |
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| عنوان انگلیسی |
Correlation Between ISAba1 Upstream ampC Gene and Resistance to Cefotaxime in Acinetobacter baumannii: A Serious Threat to Nosocomial Infections |
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| چکیده انگلیسی مقاله |
Conclusions ISAba1 has become an important factor in A. baumannii’s resistance to cefotaxime. Results ISAba1 located upstream of blaampC was found in 24 (48%) of the A. baumannii isolates. In all of the studied isolates that had ISAmpC, the MIC for cefotaxime was 64 - 256 μg/mL. Based on the REP-PCR patterns among the resistant isolates, the highest number of ISAmpC positive isolates belonged to type B (n = 19) and type C (n = 12). Objectives We examined the prevalence ISAmpC and its correlation with cefotaxime resistance. Materials and Methods Standard biochemical tests were used to identify isolates. Genomic species of the genus Acinetobacter were confirmed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). The susceptibility of 50 A. baumannii isolates to a variety of antimicrobial agents was determined using the disk diffusion method and E-test strips. PCR was used to investigate the connection of insertion sequences and the ampC gene. Clonal relatedness was determined by Repetitive Extragenic Palindromic PCR. Background Infections due to Acinetobacter baumannii have become a significant challenge in modern healthcare systems. The global upsurge of multidrug resistance in A. baumannii has created widespread problems in the treatment of patients. |
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| کلیدواژه‌های انگلیسی مقاله |
ISAba1,ampC,Nosocomial Infection,Acinetobacter baumannii |
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| نویسندگان مقاله |
دلسوز رضایی | delsuz rezaee
department of biology, faculty of natural sciences, university of tabriz, tabriz, ir iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
غلامرضا زرینی | gholamreza zarrini
department of biology, faculty of natural sciences, university of tabriz, tabriz, ir iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
محمد آهنگرزاده رضایی | mohammad ahangarzadeh rezaee
immunology research center, tabriz university of medical sciences, tabriz, ir iran; immunology research center, tabriz university of medical sciences, tabriz, ir iran. tel fax 98-4133364661
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=32417 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276848.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-32417 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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