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Avicenna Journal of Clinical Microbiology and Infection، جلد ۳، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Detection of hblA and bal Genes in Bacillus cereus Isolates From Cheese Samples Using the Polymerase Chain Reaction |
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| چکیده فارسی مقاله |
Materials and Methods Two-hundred pasteurized (n = 100) and nonpasteurized (n = 100) cheese samples were collected. The initial isolation was performed on PEMBA specific medium. Antibiotic susceptibility testing was performed using several antibiotic disks, according to the guidelines of the Clinical Laboratory and Standards Institute. Specific primers amplifying the hblA enterotoxin-encoding gene and bal hemolysin-encoding gene were used for the molecular detection of the toxins. Results Ten samples were positive for the presence of B. cereus, with both Gram staining and biochemical reactions. All the isolates were resistant to penicillin and ampicillin but susceptible to vancomycin, erythromycin, and ciprofloxacin. Six and three isolates were resistant to tetracycline and trimethoprim-sulfamethoxazole, respectively. The hblA and bal genes were amplified in all the B. cereus isolates. Conclusions The prevalence of B. cereus among the cheese samples was low. All the isolates were positive for genes encoding the hblA enterotoxin and bal toxin. Background Bacillus cereus is a Gram-positive spore-forming bacterium, which causes food poisoning. Spores enable the persistence of B. cereus in the environment, and B. cereus strains can tolerate adverse environmental conditions, such as temperature and insufficient nutrients. B. cereus causes food poisoning via the production of two enterotoxins. Most isolates produce toxins leading to diarrhea (enterotoxins) and vomiting (emetic forms). Diarrhea is caused by the production of three different heat-labile enterotoxins: HBL, NHE, and cytotoxin K. A heat-stable toxin, cereulide, is responsible for emesis. Objectives This study aimed to detect enterotoxigenic B. cereus isolates in cheese samples using the polymerase chain reaction (PCR). |
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| کلیدواژه‌های فارسی مقاله |
Hemolysin،Enterotoxin Gene،PCR،Bacillus cereus |
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| عنوان انگلیسی |
Detection of hblA and bal Genes in Bacillus cereus Isolates From Cheese Samples Using the Polymerase Chain Reaction |
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| چکیده انگلیسی مقاله |
Materials and Methods Two-hundred pasteurized (n = 100) and nonpasteurized (n = 100) cheese samples were collected. The initial isolation was performed on PEMBA specific medium. Antibiotic susceptibility testing was performed using several antibiotic disks, according to the guidelines of the Clinical Laboratory and Standards Institute. Specific primers amplifying the hblA enterotoxin-encoding gene and bal hemolysin-encoding gene were used for the molecular detection of the toxins. Results Ten samples were positive for the presence of B. cereus, with both Gram staining and biochemical reactions. All the isolates were resistant to penicillin and ampicillin but susceptible to vancomycin, erythromycin, and ciprofloxacin. Six and three isolates were resistant to tetracycline and trimethoprim-sulfamethoxazole, respectively. The hblA and bal genes were amplified in all the B. cereus isolates. Conclusions The prevalence of B. cereus among the cheese samples was low. All the isolates were positive for genes encoding the hblA enterotoxin and bal toxin. Background Bacillus cereus is a Gram-positive spore-forming bacterium, which causes food poisoning. Spores enable the persistence of B. cereus in the environment, and B. cereus strains can tolerate adverse environmental conditions, such as temperature and insufficient nutrients. B. cereus causes food poisoning via the production of two enterotoxins. Most isolates produce toxins leading to diarrhea (enterotoxins) and vomiting (emetic forms). Diarrhea is caused by the production of three different heat-labile enterotoxins: HBL, NHE, and cytotoxin K. A heat-stable toxin, cereulide, is responsible for emesis. Objectives This study aimed to detect enterotoxigenic B. cereus isolates in cheese samples using the polymerase chain reaction (PCR). |
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| کلیدواژه‌های انگلیسی مقاله |
Hemolysin,Enterotoxin Gene,PCR,Bacillus cereus |
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| نویسندگان مقاله |
شبنم مولایی کهنه شهری | shabnam molayi kohneshahri
department of microbiology, zanjan branch, faculty of basic and medical sciences, islamic azad university, zanjan, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
زهرا دیلمی خیابانی | zahra deilami khiabani
department of microbiology, zanjan branch, faculty of basic and medical sciences, islamic azad university, zanjan, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
عبدالمجید قاسمیان | abdolmajid ghasemian
microbiology department, faculty of medicine, aja university of medical sciences, tehran, ir iran
رضا شاپوری | reza shapoury
department of microbiology, zanjan branch, faculty of basic and medical sciences, islamic azad university, zanjan, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
جاوید تقی نژاد | javid taghinejad
department of microbiology, malekan branch, islamic azad university, malekan, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
مجید اسلامی | majid eslami
microbiology department, faculty of medicine, aja university of medical sciences, tehran, ir iran
سیامک حیدرزاده | siamak heidarzadeh
department of pathobiology, school of public health, tehran university of medical sciences, tehran, ir iran; department of pathobiology, school of public health, tehran university of medical sciences, qods st, poursina st, tehran, ir iran. tel 98-2188994823
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=36033 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276854.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-36033 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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