|
|
Avicenna Journal of Clinical Microbiology and Infection، جلد ۳، شماره ۳، صفحات ۰-۰
|
|
|
| عنوان فارسی |
The Prevalence of Brucella Biotypes Isolated From Sterile Body Fluids of Patients With Brucellosis in Kashan, Iran in ۲۰۱۳ |
|
| چکیده فارسی مقاله |
Results Fifty (24.3%) of the 206 samples were culture positive. SH3 production was not detected in any of the isolates, and none of the isolated strains required CO2. The results of the sensitivity test to basic fuchsin and thionin staining and specific agglutination and phage lysis (phage typing) tests indicated that all the isolated strains were biotype 1 B. melitansis. Conclusions The cause of human brucellosis in Kashan and its suburbs was biotype 1 B. melitensis. The identification of various biotypes of Brucella is important. Similar studies should be performed to detect the presence of new biotypes originating from neighboring countries. Background Brucella species are classified based on their pathogenic and genetic properties and hosts. Considering the significance of identifying different biotypes of Brucella from the epidemiological point of view and lack of such information in the city of Kashan, Iran. Objectives This study was designed to determine the biotypes and strains of Brucella isolated from patients with brucellosis. Methods This was a descriptive study of 206 samples obtained from patients with suspected brucellosis in 2013 in Kashan. BACTEC 9050 culture media was employed to test the samples. Suspected colonies of Brucella were identified through morphology, staining, and biochemical tests. The biotypes were identified by the Razi Research Institute. Lysis tests with the Tbilisi (Tb) phage were performed, the need for CO2, SH2 production, sensitivity to basic fuchsin and thionin stains, and the reaction of all the samples to specific antiserum A and M (monospecific) were tested. |
|
| کلیدواژه‌های فارسی مقاله |
Brucella،Biotype،Prevalence |
|
| عنوان انگلیسی |
The Prevalence of Brucella Biotypes Isolated From Sterile Body Fluids of Patients With Brucellosis in Kashan, Iran in 2013 |
|
| چکیده انگلیسی مقاله |
Results Fifty (24.3%) of the 206 samples were culture positive. SH3 production was not detected in any of the isolates, and none of the isolated strains required CO2. The results of the sensitivity test to basic fuchsin and thionin staining and specific agglutination and phage lysis (phage typing) tests indicated that all the isolated strains were biotype 1 B. melitansis. Conclusions The cause of human brucellosis in Kashan and its suburbs was biotype 1 B. melitensis. The identification of various biotypes of Brucella is important. Similar studies should be performed to detect the presence of new biotypes originating from neighboring countries. Background Brucella species are classified based on their pathogenic and genetic properties and hosts. Considering the significance of identifying different biotypes of Brucella from the epidemiological point of view and lack of such information in the city of Kashan, Iran. Objectives This study was designed to determine the biotypes and strains of Brucella isolated from patients with brucellosis. Methods This was a descriptive study of 206 samples obtained from patients with suspected brucellosis in 2013 in Kashan. BACTEC 9050 culture media was employed to test the samples. Suspected colonies of Brucella were identified through morphology, staining, and biochemical tests. The biotypes were identified by the Razi Research Institute. Lysis tests with the Tbilisi (Tb) phage were performed, the need for CO2, SH2 production, sensitivity to basic fuchsin and thionin stains, and the reaction of all the samples to specific antiserum A and M (monospecific) were tested. |
|
| کلیدواژه‌های انگلیسی مقاله |
Brucella,Biotype,Prevalence |
|
| نویسندگان مقاله |
مهزاد ارمی | mahzad erami
beheshti hospital, kashan university of medical sciences, kashan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)
منصوره مومن هروی | mansooreh momen heravi
department of infectious disease, social determinants of health sdh research center, kashan university of medical sciences, kashan, ir iran; department of infectious disease, social determinants of health sdh research center, kashan university of medical sciences, kashan, ir iran. tel 98-3155440459
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)
رضا رزاقی | reza razzaghi
department of infectious disease, faculty of medicine, kashan university of medical sciences, kashan, ir.iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)
سعید عالمیان | saeed alamian
depatment of brucellosis, razi serum and vaccine producing research institute, karaj, ir iran
|
|
| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=33516 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276859.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-33516 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
research-article |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|