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Avicenna Journal of Clinical Microbiology and Infection، جلد ۱، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Inhibitory Effect of Lactobacillus reuteri on Some Pathogenic Bacteria Isolated From Women With Bacterial Vaginosis |
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| چکیده فارسی مقاله |
Background Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. Objectives The purpose of this study was to investigate the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria isolated from women with bacterial vaginosis. Materials and Methods Ninety-six samples were obtained from vaginal discharge of women with bacterial vaginosis by a gynecologist with a Dacron swab and put in sterile tubes containing TSB broth and Thioglycollate broth. Then were immediately sent to the laboratory in cold chain for further assessment. Afterward, culture was transferred on blood agar, EMB, Palcam and differential diagnosis environments. Then cultures were incubated for 24 hours at 37 °C. Lactobacillus reuteri strains were cultured in MRS environment and transferred to laboratory. After purification of pathogenic bacteria, Lactobacillus reuteri inhibitory effect on pathogenic bacteria was evaluated by minimum inhibitory concentration (MIC) and antibiogram. Statistical analysis was performed using SPSS software v.16. Results The results of this study demonstrated the inhibitory effect of Lactobacillus reuteri on some pathogenic bacteria that cause bacterial, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Enterococcus, Listeria monocytogenes and E. coli. Microscopic examination of stained smears of most Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use, contraceptive methods and douching were 61%, 55%, 42% and 13%, respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial. Conclusions Our findings indicated the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria that cause bacterial vaginosis. The results of this study confirmed the inhibitory effect of probiotics on pathogens growth that cause bacterial vaginosis, which can be considered in the prevention and treatment of bacterial vaginosis in further investigations. |
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| کلیدواژه‌های فارسی مقاله |
Lactobacillus reuteri،probiotics،bacterial |
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| عنوان انگلیسی |
Inhibitory Effect of Lactobacillus reuteri on Some Pathogenic Bacteria Isolated From Women With Bacterial Vaginosis |
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| چکیده انگلیسی مقاله |
Background Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. Objectives The purpose of this study was to investigate the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria isolated from women with bacterial vaginosis. Materials and Methods Ninety-six samples were obtained from vaginal discharge of women with bacterial vaginosis by a gynecologist with a Dacron swab and put in sterile tubes containing TSB broth and Thioglycollate broth. Then were immediately sent to the laboratory in cold chain for further assessment. Afterward, culture was transferred on blood agar, EMB, Palcam and differential diagnosis environments. Then cultures were incubated for 24 hours at 37 °C. Lactobacillus reuteri strains were cultured in MRS environment and transferred to laboratory. After purification of pathogenic bacteria, Lactobacillus reuteri inhibitory effect on pathogenic bacteria was evaluated by minimum inhibitory concentration (MIC) and antibiogram. Statistical analysis was performed using SPSS software v.16. Results The results of this study demonstrated the inhibitory effect of Lactobacillus reuteri on some pathogenic bacteria that cause bacterial, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Enterococcus, Listeria monocytogenes and E. coli. Microscopic examination of stained smears of most Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use, contraceptive methods and douching were 61%, 55%, 42% and 13%, respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial. Conclusions Our findings indicated the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria that cause bacterial vaginosis. The results of this study confirmed the inhibitory effect of probiotics on pathogens growth that cause bacterial vaginosis, which can be considered in the prevention and treatment of bacterial vaginosis in further investigations. |
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| کلیدواژه‌های انگلیسی مقاله |
Lactobacillus reuteri,probiotics,bacterial |
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| نویسندگان مقاله |
گیتا اسلامی | gita eslami
department of microbiology, faculty of medicine, shahid beheshti university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
راحله کریمی روش | raheleh karimiravesh
department of microbiology, faculty of medicine, shahid beheshti university, tehran, ir iran; department of microbiology, faculty of medicine, shahid beheshti university, tehran, ir iran. tel 98-939-2206484
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
سودابه طاهری | sudabeh taheri
department of microbiology, faculty of medicine, shahid beheshti university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
اذن اله آذرگشب | eznollah azargashb
department of community medicine, faculty of medicine, shahid beheshti university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=19908 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276904.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-19908 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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