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Avicenna Journal of Clinical Microbiology and Infection، جلد ۱، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Comparison of Real-Time Polymerase Chain Reaction and Conventional Cell Culture for Detection of Influenza A in Tabriz, Iran |
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| چکیده فارسی مقاله |
Background Influenza type A (H1N1) causes an epidemic disease, resulting in thousands of deaths throughout the world. Objectives Our aim was comparing the efficacy of two different methods in isolating the Influenza (H1N1) virus including cell culture and real-Time PCR in Tabriz and suburbs. Patients and Methods Of throat swab samples, 220 were collected in viral transport medium (VTM) from patients with a suspected influenza virus infection referred to hospitals and clinics of Tabriz University of Medical Sciences. All the samples were examined through virus culturing in Madin-Darby canine kidney epithelial cells (MDCK line) and also real-time polymerase chain reaction (RT-PCR). The collected data were analyzed by SPSS version 16. Results RT-PCR detected 41 cases of influenza A (H1N1), compared with the virus isolation methods that detected 18 cases. Conclusions RT-PCR provided a sensitive and specific route compared with virus isolation. In addition, urgent planning for the vaccination program of influenza A is suggested, which can definitely prevent the spread of virus in this part of Iran. |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Comparison of Real-Time Polymerase Chain Reaction and Conventional Cell Culture for Detection of Influenza A in Tabriz, Iran |
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| چکیده انگلیسی مقاله |
Background Influenza type A (H1N1) causes an epidemic disease, resulting in thousands of deaths throughout the world. Objectives Our aim was comparing the efficacy of two different methods in isolating the Influenza (H1N1) virus including cell culture and real-Time PCR in Tabriz and suburbs. Patients and Methods Of throat swab samples, 220 were collected in viral transport medium (VTM) from patients with a suspected influenza virus infection referred to hospitals and clinics of Tabriz University of Medical Sciences. All the samples were examined through virus culturing in Madin-Darby canine kidney epithelial cells (MDCK line) and also real-time polymerase chain reaction (RT-PCR). The collected data were analyzed by SPSS version 16. Results RT-PCR detected 41 cases of influenza A (H1N1), compared with the virus isolation methods that detected 18 cases. Conclusions RT-PCR provided a sensitive and specific route compared with virus isolation. In addition, urgent planning for the vaccination program of influenza A is suggested, which can definitely prevent the spread of virus in this part of Iran. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
سیروس جداری سیفی | sirus jedary seifi
department of microbiology, faculty of medicine, tabriz university of medical sciences, tabriz, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
مسعود صبوری قناد | masoud sabouri ghannad
research center for molecular medicine, department of microbiology, research center for molecular medicine, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran; research center for molecular medicine, department of microbiology, research center for molecular medicine, faculty of medicine, hamadan university of medical sciences, hamadan, p.o.box 6517838736, ir iran. tel 98-8138380160, fax 98-8138380208
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=21034 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276905.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-21034 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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