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Journal of Research in Health Sciences، جلد ۹، شماره ۲، صفحات ۴۸-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Identification of Leishmania Species Isolated from Human Cutaneous Leishmaniasis Using PCR Method |
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| چکیده انگلیسی مقاله |
Background: To determine the epidemiological status of cutaneous leishmaniasis outbreak, isolation and identification of the agent parasite, Leishmania , using PCR method in Gonbad-e Qabus County, north Iran, during 2006-2007. Methods : Data were collected on the prevalence of scars and ulcers over a period of 3 months among 6990 inhabitants of five villages around Gonbad-e Qabus County, north Iran, during 2006-2007. Cultured promastigotes were identified using PCR technique. Its1 and its2 of Non Coding Transcribed region at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were separated by electrophoresis in 1.5% agarose gel (200 mA, 140 V), visualized by staining with ethidium bromide, and photographed. Results : Among 6990 inhabitants of 5 villages, 62.9% were identified as scars and 1.5% as active lesions. Individuals 11 to 20 years were the most highly infected age group. All the parasite isolates were Leishmania major . Conclusion : Cutaneous leishmaniasis due to L. major is endemic in Gonbad-e Qabus County, north Iran. |
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| کلیدواژه‌های انگلیسی مقاله |
Cutaneous leishmaniasis, ITS-PCR, Epidemiology, Iran |
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| نویسندگان مقاله |
n رهباریان | n rahbarian
a مسگریان | a mesgarian
m محمودی راد | m mahmoudi rad
h حجاران | h hajaran
f شهبازی | f shahbazi
z مسگریان | z mesgarian
n تقی پور | n taghipour
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| نشانی اینترنتی |
http://journals.umsha.ac.ir/index.php/JRHS/article/view/251 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Articles |
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