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Avicenna Journal of Dental Research، جلد ۸، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
Effect of Calcitriol on Differentiation of Periodontal Ligament Stem Cells to Osteoblasts |
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| چکیده فارسی مقاله |
Background Periodontium may be able to respond to injuries by regeneration via the function of stem cells. Objectives This study sought to assess the differentiation of human periodontal ligament stem cells (PDLSCs) into osteoblasts in standard osteogenic medium and in a medium supplemented with 1,25-dihydroxyvitamin D3 (calcitriol). Materials and Methods In this experimental study, PDLSCs were isolated under sterile conditions by scraping the periodontal ligament tissues attached to the middle third of the root surface of extracted teeth, which were obtained from patients who were candidates for orthodontics therapy in the dental faculty at Hamadan University. The collected cells were cultured on four culture plates for 24 hours. Group 1 contained a basic medium (α-MEM, containing 10% fetal bovine serum (FBS), 5 mM β-glycerophosphate, and 50 μg/mL l-ascorbic acid), supplemented with 10 - 8 M dexamethasone. Group 2 contained a basic medium supplemented with vitamin D3. Group 3 contained a basic medium supplemented with vitamin D3 and dexamethasone, Group4 contained negative control cultures. Alizarin red staining (ARS), alkaline phosphatase (ALP) activity, and calcium content (CC) tests were performed to evaluate osteogenic differentiation of third passage cells in the developing adherent layer. Results Quantitative analysis of ARS demonstrated that mineralized nodule formation was highest in the group supplemented with calcitriol and dexamethasone (P < 0.001). Results of the ALP test on day 28 demonstrated the highest ALP activity in the group supplemented with calcitriol (P < 0.001). The amount of CC was lowest in the control group at all-time points, and was highest in the group supplemented with both calcitriol and dexamethasone on day 28 (P < 0.001). Conclusions The combination of calcitriol with dexamethasone, ascorbic acid, and beta-glycerophosphate (that is, the osteogenic medium) may be beneficial for differentiation of PDLSCs into osteoblasts. |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Effect of Calcitriol on Differentiation of Periodontal Ligament Stem Cells to Osteoblasts |
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| چکیده انگلیسی مقاله |
Background Periodontium may be able to respond to injuries by regeneration via the function of stem cells. Objectives This study sought to assess the differentiation of human periodontal ligament stem cells (PDLSCs) into osteoblasts in standard osteogenic medium and in a medium supplemented with 1,25-dihydroxyvitamin D3 (calcitriol). Materials and Methods In this experimental study, PDLSCs were isolated under sterile conditions by scraping the periodontal ligament tissues attached to the middle third of the root surface of extracted teeth, which were obtained from patients who were candidates for orthodontics therapy in the dental faculty at Hamadan University. The collected cells were cultured on four culture plates for 24 hours. Group 1 contained a basic medium (α-MEM, containing 10% fetal bovine serum (FBS), 5 mM β-glycerophosphate, and 50 μg/mL l-ascorbic acid), supplemented with 10 - 8 M dexamethasone. Group 2 contained a basic medium supplemented with vitamin D3. Group 3 contained a basic medium supplemented with vitamin D3 and dexamethasone, Group4 contained negative control cultures. Alizarin red staining (ARS), alkaline phosphatase (ALP) activity, and calcium content (CC) tests were performed to evaluate osteogenic differentiation of third passage cells in the developing adherent layer. Results Quantitative analysis of ARS demonstrated that mineralized nodule formation was highest in the group supplemented with calcitriol and dexamethasone (P < 0.001). Results of the ALP test on day 28 demonstrated the highest ALP activity in the group supplemented with calcitriol (P < 0.001). The amount of CC was lowest in the control group at all-time points, and was highest in the group supplemented with both calcitriol and dexamethasone on day 28 (P < 0.001). Conclusions The combination of calcitriol with dexamethasone, ascorbic acid, and beta-glycerophosphate (that is, the osteogenic medium) may be beneficial for differentiation of PDLSCs into osteoblasts. |
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| نویسندگان مقاله |
سارا سهیلی فر | sara soheilifar
department of priodontics, faculty of dentistry, hamadan university of medical sciences, hamadan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
ایرج امیری | iraj amiri
research center for endometrium and endometriosis, hamadan university of medical sciences, hamadan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
محسن بیدگلی | mohsen bidgoli
department of priodontics, faculty of dentistry, hamadan university of medical sciences, hamadan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
مراد hedayatipanah | morad hedayatipanah
department of priodontics, faculty of dentistry, hamadan university of medical sciences, hamadan, ir iran; department of priodontics, faculty of dentistry, hamadan university of medical sciences, hamadan, ir iran. tel 98-9188413990
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
ژانت مرادی حقگو | janet moradi haghgoo
department of priodontics, faculty of dentistry, hamadan university of medical sciences, hamadan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
محمد علی مومنی | mohammad ali momeni
department of orthodontics, faculty of dentistry, hamadan university of medical science, hamadan, ir iran
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| نشانی اینترنتی |
http://www.avicennajdr.com/index.php?page=article&article_id=29271 |
| فایل مقاله |
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| کد مقاله (doi) |
10.17795/ajdr-29271 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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