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Avicenna Journal of Medical Biochemistry، جلد ۴، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-۶۳ and Saos Osteoblast-Like Cells |
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| چکیده فارسی مقاله |
Conclusions MG-63 and SaOS-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts. Background A large number of phospholipase C (PLC) enzymes, both mRNA transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. MG-63 and SaOS-2 human osteosarcoma cell lines are actually considered osteoblast-like cells, and are therefore widely used as experimental models for osteoblasts. Objectives Our aim was to verify whether MG-63 or SaOS-2 cells might also represent appropriate experimental osteoblast models for signal transduction studies, with special regard to the phosphoinositide (PI) pathway. We analyzed the expression and the subcellular distribution of enzymes related to calcium signal transduction (the PI-specific PLC family), which are known to possess high cell/tissue specificity. Materials and Methods The expression of PLC genes was analyzed by performing RT-PCR experiments. The presence of PLC enzymes and their subcellular distribution within the cells was analyzed with immunofluorescence experiments. Results Osteoblasts, MG-63 cells, and SaOS-2 cells have expression panels similar to those of PLC enzymes. However, slight differences were found in the expression of enzymes belonging to the PLCη subfamily. |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells |
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| چکیده انگلیسی مقاله |
Conclusions MG-63 and SaOS-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts. Background A large number of phospholipase C (PLC) enzymes, both mRNA transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. MG-63 and SaOS-2 human osteosarcoma cell lines are actually considered osteoblast-like cells, and are therefore widely used as experimental models for osteoblasts. Objectives Our aim was to verify whether MG-63 or SaOS-2 cells might also represent appropriate experimental osteoblast models for signal transduction studies, with special regard to the phosphoinositide (PI) pathway. We analyzed the expression and the subcellular distribution of enzymes related to calcium signal transduction (the PI-specific PLC family), which are known to possess high cell/tissue specificity. Materials and Methods The expression of PLC genes was analyzed by performing RT-PCR experiments. The presence of PLC enzymes and their subcellular distribution within the cells was analyzed with immunofluorescence experiments. Results Osteoblasts, MG-63 cells, and SaOS-2 cells have expression panels similar to those of PLC enzymes. However, slight differences were found in the expression of enzymes belonging to the PLCη subfamily. |
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| نویسندگان مقاله |
vincenza ریتا lo vasco | vincenza rita lo vasco
department of sensory organs, sapienza university of rome, rome, italy; department of sensory organs, sapienza university of rome, viale del policlinico, 33-00161 rome, italy
martina leopizzi | martina leopizzi
department of medico-surgical sciences and biotechnology, university of rome, rome, italy
آنا scotto d amp amp 0 abusco | anna scotto d amp amp 0 abusco
department of biochemical sciences, sapienza university of rome, rome, italy
carlo della rocca | carlo della rocca
department of medico-surgical sciences and biotechnology, university of rome, rome, italy
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=34104 |
| فایل مقاله |
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| کد مقاله (doi) |
10.17795/ajmb-34104 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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