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Avicenna Journal of Medical Biochemistry، جلد 1، شماره 1، صفحات 1-6
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| عنوان فارسی |
Effect of flaxseed on serum lipid profile and expression of NPC1L1, ABCG5 and ABCG8 genes in the intestine of diabetic rat |
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| چکیده فارسی مقاله |
Objectives The aim of this study was to examine the effect of flaxseed on gene expression of intestinal transporters: Niemann-Pick C1 like 1 (NPC1L1), ATP-cassette binding proteins G5 (ABCG5) and G8 (ABCG8). Methods Animals were randomly divided into 3 groups 8 rats in each group: group1; normal diet, group2; diabetic rats, and group3; diabetic rats + 4% (w/w) flaxseed. After one-month rats were sacrificed, blood was collected; lipid profiles were determined enzymatically, and mRNA levels were determined by RT-PCR. Results Compared to diabetic rats, flaxseed significantly decreased total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides, very low density lipoprotein cholesterol (VLDL-C) and atherogenic index (all P< 0.05). Intestinal NPC1L1 mRNA was significantly decreased (P< 0.01) in flaxseed group treatment compared with diabetic animals. Intestinal ABCG5 and ABCG8 mRNAs were significantly increased (P< 0.001) in flaxseed group treatment compared with diabetic animals. Conclusion In conclusion, flaxseed significantly reduced lipid profile and atherogenic index, as compared with the diabetic group. Flaxseed treatment also led to down-regulation of NPC1L1 mRNA and up-regulation of ABCG5 and ABCG8 mRNAs in the intestine of rats. |
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| کلیدواژه‌های فارسی مقاله |
ABCG5، ABCG8، Cholesterol، Flax، NPC1L1 |
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| عنوان انگلیسی |
Effect of flaxseed on serum lipid profile and expression of NPC1L1, ABCG5 and ABCG8 genes in the intestine of diabetic rat |
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| چکیده انگلیسی مقاله |
Objectives The aim of this study was to examine the effect of flaxseed on gene expression of intestinal transporters: Niemann-Pick C1 like 1 (NPC1L1), ATP-cassette binding proteins G5 (ABCG5) and G8 (ABCG8). Methods Animals were randomly divided into 3 groups 8 rats in each group: group1; normal diet, group2; diabetic rats, and group3; diabetic rats + 4% (w/w) flaxseed. After one-month rats were sacrificed, blood was collected; lipid profiles were determined enzymatically, and mRNA levels were determined by RT-PCR. Results Compared to diabetic rats, flaxseed significantly decreased total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides, very low density lipoprotein cholesterol (VLDL-C) and atherogenic index (all P< 0.05). Intestinal NPC1L1 mRNA was significantly decreased (P< 0.01) in flaxseed group treatment compared with diabetic animals. Intestinal ABCG5 and ABCG8 mRNAs were significantly increased (P< 0.001) in flaxseed group treatment compared with diabetic animals. Conclusion In conclusion, flaxseed significantly reduced lipid profile and atherogenic index, as compared with the diabetic group. Flaxseed treatment also led to down-regulation of NPC1L1 mRNA and up-regulation of ABCG5 and ABCG8 mRNAs in the intestine of rats. |
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| کلیدواژه‌های انگلیسی مقاله |
ABCG5, ABCG8, Cholesterol, Flax, NPC1L1 |
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| نویسندگان مقاله |
abbas mohammadi - department of biochemistry, afzalipour school of medicine, kerman university of medical sciences, kerman, iran ; physiology research centre, afzalipour school of medicine, kerman university of medical sciences, kerman, iran
fateme mirzaei - student research committee, hamadan university of medical sciences, hamadan, iran
mohammad nabi moradi - student research committee, hamadan university of medical sciences, hamadan, iran
mohammad jamshidi - student research committee, hamadan university of medical sciences, hamadan, iran
reza yari - department of biology, islamic azad university, boroūjerd branch, boroūjerd, iran
tayebeh ghiasvand - department of biochemistry and nutrition, hamadan university of medical sciences, hamadan, iran
ali rezaii - departmen of basic sciences, tehran payame noor university, tehran, iran
ebrahim abbasi oshaghi - student research committee, hamadan university of medical sciences, hamadan, iran; student research committee, hamadan university of medical sciences, hamadan, iran , 98 9126184642
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=19865 |
| فایل مقاله |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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