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Avicenna Journal of Medical Biochemistry، جلد ۱، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
In vitro investigations on the toxicity induced by tamoxifen and tamoxifen-loaded solid lipid nanoparticles on two breast cancer cell types |
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| چکیده فارسی مقاله |
Objectives Colloidal drug delivery system, solid lipid nanoparticles (SLNs), helps to increase the solubility of the drug and its oral bioavailability. Methods Tamoxifen (TAM) as a nonsteroidalantiestrogen drug was formulated in SLN and an in vitro study was conducted to determine the cytotoxicity effect of TAM-loaded SLNs on human breast cancer cell lines MCF-7 (estrogen receptor-positive) and MDA-MB231 (estrogen receptor-negative) cells. The cytotoxicity was measured by (3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay). Results The results showed that tamoxifen-loaded SLNs has an equally efficient cytotoxic activity against MCF-7 and MDA-MB231 cells, compared with free tamoxifen, and the half maximal inhibitory concentration (IC50) of TAM-loaded SLNs was generally lower than that of free TAM. Conclusion This finding indicates that tamoxifen’s cytotoxicity may result from improved drug internalization through encapsulation into the SLN matrix and endocytosis. Therefore, when TAM is incorporated into the SLN carrier system, its antitumoral activity is still preserved, suggesting that SLN is a good carrier for the drug insoluble in water. |
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| کلیدواژه‌های فارسی مقاله |
Breast Neoplasms، Nanoparticle، Tamoxifen، Toxicity |
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| عنوان انگلیسی |
In vitro investigations on the toxicity induced by tamoxifen and tamoxifen-loaded solid lipid nanoparticles on two breast cancer cell types |
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| چکیده انگلیسی مقاله |
Objectives Colloidal drug delivery system, solid lipid nanoparticles (SLNs), helps to increase the solubility of the drug and its oral bioavailability. Methods Tamoxifen (TAM) as a nonsteroidalantiestrogen drug was formulated in SLN and an in vitro study was conducted to determine the cytotoxicity effect of TAM-loaded SLNs on human breast cancer cell lines MCF-7 (estrogen receptor-positive) and MDA-MB231 (estrogen receptor-negative) cells. The cytotoxicity was measured by (3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay). Results The results showed that tamoxifen-loaded SLNs has an equally efficient cytotoxic activity against MCF-7 and MDA-MB231 cells, compared with free tamoxifen, and the half maximal inhibitory concentration (IC50) of TAM-loaded SLNs was generally lower than that of free TAM. Conclusion This finding indicates that tamoxifen’s cytotoxicity may result from improved drug internalization through encapsulation into the SLN matrix and endocytosis. Therefore, when TAM is incorporated into the SLN carrier system, its antitumoral activity is still preserved, suggesting that SLN is a good carrier for the drug insoluble in water. |
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| کلیدواژه‌های انگلیسی مقاله |
Breast Neoplasms, Nanoparticle, Tamoxifen, Toxicity |
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| نویسندگان مقاله |
رقیه عباسعلی پورکبیر | roghayeh abbasalipourkabir
department of biochemistry, faculty of medicine, hamadan university of medical science, hamadan, iran , 98 8118380572 ; department of biochemistry, faculty of medicine, hamadan university of medical science, hamadan, iran
عارف صالح زاده | aref salehzadeh
department of entomology, faculty of medicine, hamadan university of medical science, hamadan, iran
rasedee عبدالله | rasedee abdullah
department of clinical pathology and hematology, faculty of veterinary medicine, universiti putra malaysia, malaysia
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=19873 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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