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Avicenna Journal of Medical Biochemistry، جلد ۱، شماره ۱، صفحات ۴۱-۴۵
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| عنوان فارسی |
Variation of cholesterol, phospholipid and triacylglycerol content in subsets of human spermatozoa isolated by density gradient. |
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| چکیده فارسی مقاله |
Objectives Lipid components of spermatozoa have an important role in its functional activity. In this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous PureSperm gradient, was determined. Methods Aliquots of the liquefied semen samples (n=107) were layered on top of the upper layer of 40 and 80% PureSperm gradient. The resulting interfaces 40 and 80% (fraction 1), 80% and pellet (fraction 2), and pellet (fraction 3) aspirated and transferred into separate tubes. Lipids were extracted with 6 volumes of chloroform-methanol (2/1, V/V) and the concentration of cholesterol, phospholipid, triacylglycerol were determined by colorimetric method. Results Percent of sperm with normal morphology was significantly higher in sperm fraction 3 compared to fraction 2 and 1, while percent of sperm with midpiece and tail defect was significantly higher in fraction 1 compared to fractions 2 and 3 (P< 0.01). The amount of cholesterol, phospholipid and total lipid of sperm fractions 2 and 3 were about 1.5-fold higher than that of found in sperm from fraction 1 (P< 0.05). Conclusion This result suggests that the total lipid contents of sperms increase with increasing normal morphology from fraction 1 to 3 |
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| کلیدواژه‌های فارسی مقاله |
Centrifugation، Density Gradient، Lipids، Spermatozoa |
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| عنوان انگلیسی |
Variation of cholesterol, phospholipid and triacylglycerol content in subsets of human spermatozoa isolated by density gradient. |
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| چکیده انگلیسی مقاله |
Objectives Lipid components of spermatozoa have an important role in its functional activity. In this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous PureSperm gradient, was determined. Methods Aliquots of the liquefied semen samples (n=107) were layered on top of the upper layer of 40 and 80% PureSperm gradient. The resulting interfaces 40 and 80% (fraction 1), 80% and pellet (fraction 2), and pellet (fraction 3) aspirated and transferred into separate tubes. Lipids were extracted with 6 volumes of chloroform-methanol (2/1, V/V) and the concentration of cholesterol, phospholipid, triacylglycerol were determined by colorimetric method. Results Percent of sperm with normal morphology was significantly higher in sperm fraction 3 compared to fraction 2 and 1, while percent of sperm with midpiece and tail defect was significantly higher in fraction 1 compared to fractions 2 and 3 (P< 0.01). The amount of cholesterol, phospholipid and total lipid of sperm fractions 2 and 3 were about 1.5-fold higher than that of found in sperm from fraction 1 (P< 0.05). Conclusion This result suggests that the total lipid contents of sperms increase with increasing normal morphology from fraction 1 to 3 |
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| کلیدواژه‌های انگلیسی مقاله |
Centrifugation, Density Gradient, Lipids, Spermatozoa |
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| نویسندگان مقاله |
روح اله ستاره بادی | rohollah setarehbadi
student research committee, hamadan university of medical sciences, hamadan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
مژگان atabakhash | mojgan atabakhash
student research committee, hamadan university of medical sciences, hamadan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
امیر فتاحی | amir fattahi
student research committee, hamadan university of medical sciences, hamadan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
ابوذر محققی | aboozar mohagheghi
student research committee, hamadan university of medical sciences, hamadan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
اسد ویسی رایگانی | asad vaisi raygani
fertility and infertility center, kermanshah university of medical sciences, kermanshah, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)
حسین محجوب | hossain mahjub
department of epidemiology and biostatistics, faculty of public health, hamadan university of medical science, hamadan, iran
حیدر طویلانی طویلانی | heidar tavilani tavilani
urology amp;amp; nephrology research center, hamadan university of medical sciences, hamadan iran , 98 8118380717; urology amp;amp; nephrology research center, hamadan university of medical sciences, hamadan iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
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| نشانی اینترنتی |
http://www.avicennajb.com/index.php?page=article&article_id=19870 |
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| زبان مقاله منتشر شده |
fa |
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| نوع مقاله منتشر شده |
research-article |
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