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Avicenna Journal of Clinical Microbiology and Infection، جلد ۱، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
Prevalence and Resistance Profiles of Enteropathogenic and Shiga Toxin-Producing Escherichia coli in Diarrheic Calves in Mashhad and Garmsar Districts, Iran |
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| چکیده فارسی مقاله |
Background Shiga toxin-producing Escherichia coli (STEC) strains are considered as one of the most important widespread food-borne pathogens, which cause diarrhea and life threatening diseases, such as hemolytic uremic syndrome, in humans. More recently, the STEC strains have also been incriminated to cause diarrhea and hemorrhagic colitis in calves; enteropathogenic E. coli (EPEC) also causes diarrhea in neonate animals. Objectives This study aimed to study the prevalence and antibacterial resistance patterns of STEC and EPEC in fecal samples from diarrheic calves in Mashhad and Garmsar districts, Iran. Materials and Methods A total of 115 fecal samples were collected from diarrheic animals, 75 from Mashhad and 40 from Garmsar districts. A total of 146 E. coli isolates were obtained from culture and subjected to multiplex-PCR assay targeting stx1, stx2, eaeA and ehly virulence genes. The antibacterial resistance patterns of the virulence-positive isolates were determined using disc diffusion method. Results Eight samples (6.9%) carried the strains with positive results for at least one of the tested virulence genes. Five samples (4.3%) contained the stx-positive strains (STEC) and three (2.6%) carried the eaeA-positive and stx-negative strains, which were categorized as EPEC. In nine virulence-positive E. coli isolates, stx1 (n = 6) was the predominant virulence gene, followed by ehly (n = 5), eae (n = 4), and stx2 (n = 2). Antibacterial resistance patterns of virulence-positive isolates were also determined and nine resistance profiles were discriminated; higher rates of resistance were observed in isolates from Mashhad. Conclusions This study indicated that other pathologic factors might play a more important role in calf diarrhea in the studied areas, but public health significance of these strains should not be overlooked. |
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| کلیدواژه‌های فارسی مقاله |
STEC،EPEC،Calf Diarrhea،Antibacterial Resistance |
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| عنوان انگلیسی |
Prevalence and Resistance Profiles of Enteropathogenic and Shiga Toxin-Producing Escherichia coli in Diarrheic Calves in Mashhad and Garmsar Districts, Iran |
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| چکیده انگلیسی مقاله |
Background Shiga toxin-producing Escherichia coli (STEC) strains are considered as one of the most important widespread food-borne pathogens, which cause diarrhea and life threatening diseases, such as hemolytic uremic syndrome, in humans. More recently, the STEC strains have also been incriminated to cause diarrhea and hemorrhagic colitis in calves; enteropathogenic E. coli (EPEC) also causes diarrhea in neonate animals. Objectives This study aimed to study the prevalence and antibacterial resistance patterns of STEC and EPEC in fecal samples from diarrheic calves in Mashhad and Garmsar districts, Iran. Materials and Methods A total of 115 fecal samples were collected from diarrheic animals, 75 from Mashhad and 40 from Garmsar districts. A total of 146 E. coli isolates were obtained from culture and subjected to multiplex-PCR assay targeting stx1, stx2, eaeA and ehly virulence genes. The antibacterial resistance patterns of the virulence-positive isolates were determined using disc diffusion method. Results Eight samples (6.9%) carried the strains with positive results for at least one of the tested virulence genes. Five samples (4.3%) contained the stx-positive strains (STEC) and three (2.6%) carried the eaeA-positive and stx-negative strains, which were categorized as EPEC. In nine virulence-positive E. coli isolates, stx1 (n = 6) was the predominant virulence gene, followed by ehly (n = 5), eae (n = 4), and stx2 (n = 2). Antibacterial resistance patterns of virulence-positive isolates were also determined and nine resistance profiles were discriminated; higher rates of resistance were observed in isolates from Mashhad. Conclusions This study indicated that other pathologic factors might play a more important role in calf diarrhea in the studied areas, but public health significance of these strains should not be overlooked. |
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| کلیدواژه‌های انگلیسی مقاله |
STEC,EPEC,Calf Diarrhea,Antibacterial Resistance |
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| نویسندگان مقاله |
مهدی عسکری badouei | mahdi askari badouei
department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar, ir iran; department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar, ir iran. tel 98-2334252121, fax 98-2334252020
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
صمد لطف اله زاده | samad lotfollahzadeh
department of large animal internal medicine, faculty of veterinary medicine, university of tehran, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
معین آرمان | moein arman
faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
مسعود حدادی | masoud haddadi
faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar, ir iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=22802 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276840.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-22802 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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