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Avicenna Journal of Clinical Microbiology and Infection، جلد ۱، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B۲ and A Escherichia coli Isolates |
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| چکیده فارسی مقاله |
Background Escherichia coli is one of the most causative pathogen of urinary tract infection. Urinary tract infections (UTIs) are the second most common cause of morbidity and remain a serious health concern among the clinicians. The severity of UTI caused by uropathogenic E. coli (UPEC) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. Little is known about the relationship between the E. coli genetic background and the acquisition of adhesin coding operons in UPEC isolates. Objectives The aim of this study was to determine the prevalence of adhesin coding operons in UPEC isolates belonged to phylogenetic group B2 and A collected from patients suffering from UTI. Materials and Methods A total 100 UPEC isolates were used for DNA extraction by the boiling lysis. The analysis of phylogenetic groups, along with detection of adhesin coding operons was performed by Multiplex-PCR method. Associations were assessed between afa, fim, foc, pap and sfa operons among to 55 B2 and 17 A groups E. coli isolates. Statistical analysis was performed using Fisher exact test. Results Phylogenetic analysis showed that 55 and 17 of 100 UPEC isolates belonged to the B2 and A phylogenetic groups, respectively. The afa, fim, foc, pap and sfa operons were present in five (9.09%), 55 (100%), 16 (29.09%), 46 (83.63%) and 46 (83.63%) of UPEC isolates belonged to phylogenetic group B2, and two (12.50%), 14 (87.50%), one (6.25%), two (12.5%) and 12 (75%) of isolates belonged to phylogenetic group A, respectively. Statistical analysis showed that pap gene was significantly more frequently detected in phylogenetic group B2 (P < 0.05). Conclusions The UPEC isolates belonging to group B2 harbored a greater number of adhesin coding operons than strains from phylogenetic groups A. |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates |
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| چکیده انگلیسی مقاله |
Background Escherichia coli is one of the most causative pathogen of urinary tract infection. Urinary tract infections (UTIs) are the second most common cause of morbidity and remain a serious health concern among the clinicians. The severity of UTI caused by uropathogenic E. coli (UPEC) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. Little is known about the relationship between the E. coli genetic background and the acquisition of adhesin coding operons in UPEC isolates. Objectives The aim of this study was to determine the prevalence of adhesin coding operons in UPEC isolates belonged to phylogenetic group B2 and A collected from patients suffering from UTI. Materials and Methods A total 100 UPEC isolates were used for DNA extraction by the boiling lysis. The analysis of phylogenetic groups, along with detection of adhesin coding operons was performed by Multiplex-PCR method. Associations were assessed between afa, fim, foc, pap and sfa operons among to 55 B2 and 17 A groups E. coli isolates. Statistical analysis was performed using Fisher exact test. Results Phylogenetic analysis showed that 55 and 17 of 100 UPEC isolates belonged to the B2 and A phylogenetic groups, respectively. The afa, fim, foc, pap and sfa operons were present in five (9.09%), 55 (100%), 16 (29.09%), 46 (83.63%) and 46 (83.63%) of UPEC isolates belonged to phylogenetic group B2, and two (12.50%), 14 (87.50%), one (6.25%), two (12.5%) and 12 (75%) of isolates belonged to phylogenetic group A, respectively. Statistical analysis showed that pap gene was significantly more frequently detected in phylogenetic group B2 (P < 0.05). Conclusions The UPEC isolates belonging to group B2 harbored a greater number of adhesin coding operons than strains from phylogenetic groups A. |
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| نویسندگان مقاله |
مسعود رهدار | masoud rahdar
department of biology, faculty of basic science, university of zabol, zabol, ir iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
احمد راشکی | ahmad rashki
department of physiopathology, faculty of vet-medicine, university of zabol, zabol, ir iran; department of physiopathology, faculty of vet-medicine, university of zabol, zabol, ir iran. tel 98-9151970877, fax 98-5424822251
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
حمیدرضا میری | hamidreza miri
department of biology, faculty of basic science, university of zabol, zabol, ir iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=22981 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276845.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-22981 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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