Avicenna Journal of Clinical Microbiology and Infection، جلد 3، شماره 3، صفحات 0-0
عنوان فارسی The Study of the Inhibition Effects of Satureja khuzestaniea Essence on the Gene Expression of bla-OXA-23 in Multidrug-Resistant Strains of Acinetobacter baumannii
چکیده فارسی مقاله Conclusions This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii. Background Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments. Objectives The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique. Methods Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique. Results The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene.
کلیدواژه‌های فارسی مقاله RT-PCR،bla-OXA-23،Satureja khuzestaniea
عنوان انگلیسی The Study of the Inhibition Effects of Satureja khuzestaniea Essence on the Gene Expression of bla-OXA-23 in Multidrug-Resistant Strains of Acinetobacter baumannii
چکیده انگلیسی مقاله Conclusions This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii. Background Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments. Objectives The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique. Methods Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique. Results The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene.
کلیدواژه‌های انگلیسی مقاله RT-PCR,bla-OXA-23,Satureja khuzestaniea
نویسندگان مقاله hossein saghi - department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran; aja university of medical sciences, tehran, ir iran

ferdoes amiri dastjerdi - nursing care research center, semnan university of medical sciences, semnan, ir iran

bahador zahedi - department of microbiology, faculty of medicine, mazandaran university of medical sciences, sari, ir iran

mohammad mohsen pour - department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran

mahmoud khorrami - aja university of medical sciences, tehran, ir iran

majid efati - aja university of medical sciences, tehran, ir iran

davoud esmaeili - department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran; department of microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran. tel 98-218052941

نشانی اینترنتی http://www.ajcmicrob.com/index.php?page=article&article_id=35694
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276860.pdf
کد مقاله (doi) 10.17795/ajcmi-35694
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده research-article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات