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Avicenna Journal of Clinical Microbiology and Infection، جلد ۳، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
The Study of the Inhibition Effects of Satureja khuzestaniea Essence on the Gene Expression of bla-OXA-۲۳ in Multidrug-Resistant Strains of Acinetobacter baumannii |
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| چکیده فارسی مقاله |
Conclusions This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii. Background Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments. Objectives The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique. Methods Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique. Results The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene. |
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| کلیدواژه‌های فارسی مقاله |
RT-PCR،bla-OXA-23،Satureja khuzestaniea |
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| عنوان انگلیسی |
The Study of the Inhibition Effects of Satureja khuzestaniea Essence on the Gene Expression of bla-OXA-23 in Multidrug-Resistant Strains of Acinetobacter baumannii |
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| چکیده انگلیسی مقاله |
Conclusions This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii. Background Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments. Objectives The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique. Methods Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique. Results The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene. |
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| کلیدواژه‌های انگلیسی مقاله |
RT-PCR,bla-OXA-23,Satureja khuzestaniea |
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| نویسندگان مقاله |
حسین ساقی | hossein saghi
department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran; aja university of medical sciences, tehran, ir iran
فردوس امیری دستجردی | ferdoes amiri dastjerdi
nursing care research center, semnan university of medical sciences, semnan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی سمنان (Semnan university of medical sciences)
بهادر زاهدی | bahador zahedi
department of microbiology, faculty of medicine, mazandaran university of medical sciences, sari, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
محمد محسن پور | mohammad mohsen pour
department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran
محمود خرمی | mahmoud khorrami
aja university of medical sciences, tehran, ir iran
مجید عفتی | majid efati
aja university of medical sciences, tehran, ir iran
داود اسماعیلی | davoud esmaeili
department of microbiology, baqiyatallah university medical of sciences, tehran, ir iran; department of microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran. tel 98-218052941
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=35694 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276860.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-35694 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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