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Avicenna Journal of Clinical Microbiology and Infection، جلد ۲، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
Sequence Variation of the Pertussis Toxin S۱ Subunit Encoding Gene in the Clinical Isolates of Bordetella pertussis in Iran |
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| چکیده فارسی مقاله |
Results The results showed that all the strains had the dominant allele ptxS1A. There were differences between the alleles of the clinical strains and the vaccine strain. Conclusions In recent years, a significant increase in the incidence of pertussis has been reported worldwide. Our findings regarding the allelic shift of the ptxS1 gene are similar to those reported in many European and American countries showing the difference of the dominant allele of ptxS1 between the circulating isolates and the vaccine strains. Objectives The aim of this study was to identify the polymorphisms of the pertussis toxin S1 subunit (ptxS1) gene in the circulating strains and compare them to the vaccine strain. Patients and Methods In this study, 50 strains of B. pertussis isolated from patients with pertussis were investigated in the pertussis reference laboratory of Pasteur institute of Iran. Cultivation, biochemical tests, and the specific antisera were used to confirm B. pertussis. The sequencing of the polymerase chain reaction products was performed to determine the ptxS1 alleles, and B. pertussis 134 was studied as the vaccine strain. Background Whooping cough (pertussis) is an acute respiratory disease caused by Bordetella pertussis (B. pertussis). Pertussis toxin is an important virulence factor of B. pertussis and plays a major role in the immune and inflammatory responses. Likewise, allelic variations in the genes of virulence factors have led to the non-responsiveness of the new strains to both whole-cell and acellular vaccines. Given the importance of pertussis vaccine, we sought to address the lack of fundamental studies on the polymorphisms of the virulence genes of B. pertussis in Iran. |
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| کلیدواژه‌های فارسی مقاله |
Pertussis Vaccine،Whooping Cough،Bordetella pertussis |
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| عنوان انگلیسی |
Sequence Variation of the Pertussis Toxin S1 Subunit Encoding Gene in the Clinical Isolates of Bordetella pertussis in Iran |
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| چکیده انگلیسی مقاله |
Results The results showed that all the strains had the dominant allele ptxS1A. There were differences between the alleles of the clinical strains and the vaccine strain. Conclusions In recent years, a significant increase in the incidence of pertussis has been reported worldwide. Our findings regarding the allelic shift of the ptxS1 gene are similar to those reported in many European and American countries showing the difference of the dominant allele of ptxS1 between the circulating isolates and the vaccine strains. Objectives The aim of this study was to identify the polymorphisms of the pertussis toxin S1 subunit (ptxS1) gene in the circulating strains and compare them to the vaccine strain. Patients and Methods In this study, 50 strains of B. pertussis isolated from patients with pertussis were investigated in the pertussis reference laboratory of Pasteur institute of Iran. Cultivation, biochemical tests, and the specific antisera were used to confirm B. pertussis. The sequencing of the polymerase chain reaction products was performed to determine the ptxS1 alleles, and B. pertussis 134 was studied as the vaccine strain. Background Whooping cough (pertussis) is an acute respiratory disease caused by Bordetella pertussis (B. pertussis). Pertussis toxin is an important virulence factor of B. pertussis and plays a major role in the immune and inflammatory responses. Likewise, allelic variations in the genes of virulence factors have led to the non-responsiveness of the new strains to both whole-cell and acellular vaccines. Given the importance of pertussis vaccine, we sought to address the lack of fundamental studies on the polymorphisms of the virulence genes of B. pertussis in Iran. |
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| کلیدواژه‌های انگلیسی مقاله |
Pertussis Vaccine,Whooping Cough,Bordetella pertussis |
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| نویسندگان مقاله |
مریم حسین پور | maryam hosseinpour
pertussis reference laboratory, department of bacteriology, pasteur institute, tehran, ir iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
نازنین جان نثار احمدی | nazanin jannesar ahmadi
pertussis reference laboratory, department of bacteriology, pasteur institute, tehran, ir iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
وجیهه سادات نیک بین | vajiheh sadat nikbin
pertussis reference laboratory, department of bacteriology, pasteur institute, tehran, ir iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
فرشته شاهچراغی | fereshteh shahcheraghi
pertussis reference laboratory, department of bacteriology, pasteur institute, tehran, ir iran; pertussis reference laboratory, department of bacteriology, pasteur institute, tehran, ir iran. tel fax 98-2166405535
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=21384 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276888.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-21384 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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