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Avicenna Journal of Clinical Microbiology and Infection، جلد ۲، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
Extended Spectrum β-Lactamase-Producing Strains of Escherichia coli in Hospitalized Children in Isfahan, Iran |
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| چکیده فارسی مقاله |
Conclusions Our data showed that, the high prevalence of beta-lactamase genes among isolates, and it. Our findings may provide useful insights in replace of the appropriate antibiotics and it may also prevents of ESBLs, mediated resistance problem. Results From 145 strains of E. coli, 51(35.2%) and 61(42.1%) isolates were resistance to ceftazidime and cefotaxime, respectively. ESBL production was noted in 59 (40.7%) strains. The amplification of β-lactamase genes showed the presence of blaCTX-M like genes in 22 (37.9%) strains, blaTEM in 42 (72.4%), blaSHV in 7 (11.9%) and blaOXA in 23 (39%) of the total 58 strains of E. coli. Objectives The aims of this study was evaluation of the susceptibility of the ESBL-producing E. coli strains to ordinarily used antibiotics and to detect the presence of the four common ESBL genes: blaTEM, blaOXA, blaCTX-M, and blaSHV using multiplex PCR method. Materials and Methods In a retrospective cross sectional study conducted between 2012 and 2013, out of145 strains of E. coli were isolated from hospitalized children with UTIs. Susceptibility test was performed by disc diffusion method. All isolates were also tested for ESBL by the standard CLSI double disk diffusion method, using cefotaxime/ clavulanic acid and ceftazidime/clavulanic acid disks. Multiplex PCR was performed for detect of blaCTX-MIV, blaOXA, blaTEM, and blaSHV genes. Background Beta-lactam antibiotics such as long-spectrum cephalosporins and carbapenems are the empirical treatment against urinary tract infections (UTIs). The common mechanism of resistance to B-lactamas in Escherichia coli is producing of ESBLs. |
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| کلیدواژه‌های فارسی مقاله |
Beta-Lactamase،UTI،Children،Escherichia coli |
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| عنوان انگلیسی |
Extended Spectrum β-Lactamase-Producing Strains of Escherichia coli in Hospitalized Children in Isfahan, Iran |
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| چکیده انگلیسی مقاله |
Conclusions Our data showed that, the high prevalence of beta-lactamase genes among isolates, and it. Our findings may provide useful insights in replace of the appropriate antibiotics and it may also prevents of ESBLs, mediated resistance problem. Results From 145 strains of E. coli, 51(35.2%) and 61(42.1%) isolates were resistance to ceftazidime and cefotaxime, respectively. ESBL production was noted in 59 (40.7%) strains. The amplification of β-lactamase genes showed the presence of blaCTX-M like genes in 22 (37.9%) strains, blaTEM in 42 (72.4%), blaSHV in 7 (11.9%) and blaOXA in 23 (39%) of the total 58 strains of E. coli. Objectives The aims of this study was evaluation of the susceptibility of the ESBL-producing E. coli strains to ordinarily used antibiotics and to detect the presence of the four common ESBL genes: blaTEM, blaOXA, blaCTX-M, and blaSHV using multiplex PCR method. Materials and Methods In a retrospective cross sectional study conducted between 2012 and 2013, out of145 strains of E. coli were isolated from hospitalized children with UTIs. Susceptibility test was performed by disc diffusion method. All isolates were also tested for ESBL by the standard CLSI double disk diffusion method, using cefotaxime/ clavulanic acid and ceftazidime/clavulanic acid disks. Multiplex PCR was performed for detect of blaCTX-MIV, blaOXA, blaTEM, and blaSHV genes. Background Beta-lactam antibiotics such as long-spectrum cephalosporins and carbapenems are the empirical treatment against urinary tract infections (UTIs). The common mechanism of resistance to B-lactamas in Escherichia coli is producing of ESBLs. |
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| کلیدواژه‌های انگلیسی مقاله |
Beta-Lactamase,UTI,Children,Escherichia coli |
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| نویسندگان مقاله |
مهناز کریمیان | mahnaz karimian
department of biology, faculty of science, university of ilam, ilam, ir iran
سازمان اصلی تایید شده: دانشگاه ایلام (Ilam university)
آرمان رستم زاد | arman rostamzad
department of biology, faculty of science, university of ilam, ilam, ir iran; department of biology, faculty of science, university of ilam, ilam, ir iran. tel fax 98-8432227022
سازمان اصلی تایید شده: دانشگاه ایلام (Ilam university)
پریسا شعاعی | parisa shoaei
nosocomial infection research center, isfahan university of medical sciences, isfahan, ir iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=27096 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276889.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-27096 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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