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Avicenna Journal of Clinical Microbiology and Infection، جلد ۲، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
Emergence of blaCTX-M-۱۵ Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran |
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| چکیده فارسی مقاله |
Conclusions It was the first report on the blaCTX-M-15 gene emergence in clinical Enterobacter spp. in Iran. The current study demonstrated the predominant presence of the gene encoding CTX-M-15 among ESBL producing Enterobacter spp. commonly with a large plasmid, in the setting. Background Enterobacter spp. is increasingly recognized as an important nosocomial pathogen and implicated in many episodes of hospital acquired infections. Objectives The current study aimed to describe distribution and transferability of blaCTX-M-15 gene, and the antibiotic susceptibility pattern in the clinical isolates of Enterobacter spp. Materials and Methods A total of 110 Enterobacter isolates were collected from five hospitals in Tehran, Iran from May 2012 to April 2013. Enterobacter species were identified by API 20E system. Antibacterial susceptibility was determined by disk diffusion method, and extended spectrum beta lactamase (ESBL) production was confirmed by combined disk method. The blaCTX-M-15 gene was identified by PCR with specific primers. The transferability of the blaCTX-M-15was tested by conjugation with broth matting method. Results The prevalence of Enterobacter species was E. cloacae (78.2 %), E. aerogenes (6.13 %) and E. sakazakii (8.2%). They were from different clinical sources. Maximal resistance in Enterobacter isolates was noticed against Augmentin®, trimethoprim - sulfamethoxazole and cefoxitin 75.5%, 64.5%, and 59.1%, respectively. Fourteen isolates, showed ESBL phenotype. The blaCTX-M-15 gene frequency in Enterobacter isolates was 11.8%. Three conjugative plasmids containing blaCTX-M-15 were found in one Enterobacter isolate. |
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| کلیدواژه‌های فارسی مقاله |
Conjugation،Drug Resistance،ESBLs،Enterobacter |
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| عنوان انگلیسی |
Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran |
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| چکیده انگلیسی مقاله |
Conclusions It was the first report on the blaCTX-M-15 gene emergence in clinical Enterobacter spp. in Iran. The current study demonstrated the predominant presence of the gene encoding CTX-M-15 among ESBL producing Enterobacter spp. commonly with a large plasmid, in the setting. Background Enterobacter spp. is increasingly recognized as an important nosocomial pathogen and implicated in many episodes of hospital acquired infections. Objectives The current study aimed to describe distribution and transferability of blaCTX-M-15 gene, and the antibiotic susceptibility pattern in the clinical isolates of Enterobacter spp. Materials and Methods A total of 110 Enterobacter isolates were collected from five hospitals in Tehran, Iran from May 2012 to April 2013. Enterobacter species were identified by API 20E system. Antibacterial susceptibility was determined by disk diffusion method, and extended spectrum beta lactamase (ESBL) production was confirmed by combined disk method. The blaCTX-M-15 gene was identified by PCR with specific primers. The transferability of the blaCTX-M-15was tested by conjugation with broth matting method. Results The prevalence of Enterobacter species was E. cloacae (78.2 %), E. aerogenes (6.13 %) and E. sakazakii (8.2%). They were from different clinical sources. Maximal resistance in Enterobacter isolates was noticed against Augmentin®, trimethoprim - sulfamethoxazole and cefoxitin 75.5%, 64.5%, and 59.1%, respectively. Fourteen isolates, showed ESBL phenotype. The blaCTX-M-15 gene frequency in Enterobacter isolates was 11.8%. Three conjugative plasmids containing blaCTX-M-15 were found in one Enterobacter isolate. |
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| کلیدواژه‌های انگلیسی مقاله |
Conjugation,Drug Resistance,ESBLs,Enterobacter |
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| نویسندگان مقاله |
کبری سلیمیان ریزی | kobra salimian rizi
department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran; department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran. tel 98-9382657837, fax 98-1288006544
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
شاهین نجار پیرایه | shahin najar peerayeh
department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
بیتا بخشی | bita bakhshi
department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
محمد رهبر | mohammad rahbar
department of microbiology, iranian reference health laboratory, deputy of health, ministry of health and medical education, tehran, ir iran
سازمان اصلی تایید شده: وزارت بهداشت درمان و آموزش پزشکی (Ministry of health and medical education)
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=29691 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276894.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-29691 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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