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Avicenna Journal of Clinical Microbiology and Infection، جلد 2، شماره 4، صفحات 0-0
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| عنوان فارسی |
Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection |
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| چکیده فارسی مقاله |
Conclusions Our successful extraction of purified LPS from E. coli isolates of UTI patients’ urine samples can be an important step to understand the UTI disease conditions. Results The silver-stained gel demonstrated both smooth and rough type LPS by showing trail-like band patterns with the presence and lacking O-antigen region, respectively. Coomassie blue staining showed no band assuring the absence of any contaminating protein. Patients and Methods The E. coli strain was isolated from the urine samples of 10 patients with UTI and then the antibiotic sensitivity pattern of the isolates was determined. The purification of LPS was carried out using the hot aqueous-phenol method and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, which was directly stained using the modified silver staining method and Coomassie blue. Background Urinary tract infection (UTI) is one of the most common infectious diseases in Bangladesh where Escherichia coli is the prevalent organism and responsible for most of the infections. Lipopolysaccharide (LPS) is known to act as a major virulence factor of E. coli. Objectives The present study aimed to purify, extract and visualize LPS of E. coli clinical isolates from urine samples of patients with UTI. |
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| عنوان انگلیسی |
Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection |
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| چکیده انگلیسی مقاله |
Conclusions Our successful extraction of purified LPS from E. coli isolates of UTI patients’ urine samples can be an important step to understand the UTI disease conditions. Results The silver-stained gel demonstrated both smooth and rough type LPS by showing trail-like band patterns with the presence and lacking O-antigen region, respectively. Coomassie blue staining showed no band assuring the absence of any contaminating protein. Patients and Methods The E. coli strain was isolated from the urine samples of 10 patients with UTI and then the antibiotic sensitivity pattern of the isolates was determined. The purification of LPS was carried out using the hot aqueous-phenol method and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, which was directly stained using the modified silver staining method and Coomassie blue. Background Urinary tract infection (UTI) is one of the most common infectious diseases in Bangladesh where Escherichia coli is the prevalent organism and responsible for most of the infections. Lipopolysaccharide (LPS) is known to act as a major virulence factor of E. coli. Objectives The present study aimed to purify, extract and visualize LPS of E. coli clinical isolates from urine samples of patients with UTI. |
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| نویسندگان مقاله |
fariha akhter chowdhury - department of pharmaceutical sciences, north south university, dhaka, bangladesh
mohammad nurul islam - department of pharmaceutical sciences, north south university, dhaka, bangladesh
anamika saha - department of pharmaceutical sciences, north south university, dhaka, bangladesh
sabrina mahboob - department of pharmaceutical sciences, north south university, dhaka, bangladesh
abu syed md mosaddek - department of pharmacology, uttara adhunik medical college, dhaka, bangladesh
md omar faruque - department of physiology and molecular biology, bangladesh university of health sciences, dhaka, bangladesh
most fahmida begum - department of microbiology, uttara adhunik medical college, dhaka, bangladesh
rajib bhattacharjee - department of pharmaceutical sciences, north south university, dhaka, bangladesh; department of pharmaceutical sciences, north south university, dhaka, bangladesh. tel 1-7807079527, fax 880-255668202
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| نشانی اینترنتی |
http://www.ajcmicrob.com/index.php?page=article&article_id=31990 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/1795/article-1795-276900.pdf |
| کد مقاله (doi) |
10.17795/ajcmi-31990 |
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
research-article |
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